Anti-Human FCGRT Recombinant Antibody (TAB-450CQ)
CAT#: TAB-450CQ
Recombinant monoclonal antibody to FCGRT. This antibody is a humanized monoclonal antibody. It is designed to target the neonatal Fc receptor (FcRn), a receptor that plays a crucial role in regulating the half-life and recycling of immunoglobulin G (IgG) antibodies.
Tested Data
Gene Expression
WB
Figure 1 Anti-Human FCGRT Antibody (TAB-450CQ) in WB
Western blot analysis of TAB-450CQ was performed by loading Human FCGRT Protein (His Tag) in reduced condition Lane 1, Lane 2 and Lane 3 (0.1 μg, 0.3 μg and 0.6 μg respectively) onto a 12% Tris-HCl polyacrylamide gel. Proteins were transferred to a CN membrane and blocked with 5% skim milk for at least one hour. Membranes were probed with TAB-450CQ (Left, 2 μg/mL) and TAB-450CQ-FITC (Right, 2 μg/mL). HRP-conjugated goat anti-human IgG as a secondary antibody (1: 6000). Chemiluminescent detection was performed.
DB
Figure 2 Anti-Human FCGRT Antibody (TAB-450CQ) in DB
Dot Blot analysis of TAB-450CQ was performed by coating with Human FCGRT Protein (His Tag).
TAB-450CQ (Top) and TAB-450CQ-FITC (Bottom) incubation concentration: 2 μg/mL.
HRP-conjugated goat anti-human IgG as a secondary antibody (1: 6000)
NC: Negative control PC: Positive control
ELISA
Figure 3 Anti-Human FCGRT Antibody (TAB-450CQ) in ELISA
ELISA analysis of TAB-450CQ was performed by coating with Human FCGRT Protein (His Tag). Then blocked with BSA and incubated with TAB-450CQ and TAB-450CQ-FITC. The HRP-conjugated goat anti-human IgG as a secondary antibody (1: 8000). Detection was performed using TMB substrate and stopped with sulfuric acid. The absorbances were read on a spectrophotometer at 450 nm.
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RNA Expression
Figure 1 RNA cell line category: Cell line enhanced (ASC diff, Hep G2, OE19, THP-1)
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000104870-FCGRT
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Specifications
- Immunogen
- The details of the immunogen for this antibody are not available.
- Host Species
- Human
- Derivation
- Humanized and chimeric
- Type
- IgG4, κ
- Specificity
- Human FCGRT
- Species Reactivity
- Human
- Applications
- Used for immunoassay techniques such as: Enzyme-Linked Immunosorbent Assay; Immunohistochemistry; Immunofluorescence; Immunoprecipitation; Flow Cytometry; Functional Studies
- Conjugate
- Unconjugated
- Related Disease
- Immunological diseases, Thrombocytopenia
Product Property
- Purity
- > 95% as determined by SDS-PAGE
- Storage
- Store at -20°C for long-term storage. Avoid freeze/thaw cycles.
Target
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Excellent for Flow Cytometry
This antibody works excellently in flow cytometry applications. We used it to assess FCGRT expression on different cell types, and the results were highly reliable and reproducible. The signal was strong, and nonspecific binding was minimal. The availability of custom conjugation services made it even more convenient for our multicolor panels.
Versatile Application
We've successfully used this antibody in various applications, including flow cytometry, immunohistochemistry, and immunofluorescence and immunoprecipitation. Its versatility has allowed us to explore different aspects of FCGRT function without needing multiple antibodies. This has streamlined our workflow and ensured consistent results across different experimental setups.
Q&As
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What are the main applications of the Anti-Human FCGRT Recombinant Antibody (TAB-450CQ)?
A: The Anti-Human FCGRT Recombinant Antibody (TAB-450CQ) is primarily used in research to study the expression and function of the FCGRT protein, also known as the neonatal Fc receptor (FcRn). It is suitable for applications such as flow cytometry, immunohistochemistry, ELISA, and immunoprecipitation.
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Can this antibody be used for immunohistochemistry on paraffin-embedded tissues?
A: Yes, the Anti-Human FCGRT Recombinant Antibody is suitable for immunohistochemistry (IHC) on paraffin-embedded tissue sections. It allows researchers to visualize the localization and distribution of FCGRT within various human tissues. Optimal antigen retrieval methods should be applied to achieve the best staining results. This application is valuable for studying the role of FCGRT in different physiological and pathological conditions.
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Is this antibody compatible with flow cytometry applications?
A: Absolutely, this antibody is compatible with flow cytometry. It can be used to detect and quantify FCGRT expression on the surface or within human cells. By conjugating the antibody with appropriate fluorophores or using a labeled secondary antibody, researchers can analyze FCGRT expression levels across different cell populations, aiding in studies of immune responses and antibody transport mechanisms.
View the frequently asked questions answered by Creative Biolabs Support.
Cite This Product
To accurately reference this product in your publication, please use the following citation information:
(Creative Biolabs Cat# TAB-450CQ, RRID: AB_3111951)
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Biosimilar Overview
Please refer to Rozanolixizumab Overview to learn more about the mechanism of action, clinical projects, and approved drugs of Rozanolixizumab.
Downloadable Resources
Download resources about recombinant antibody development and antibody engineering to boost your research.
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
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Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
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| CAT | Product Name | Application | Type |
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| HPAB-0199-CN-F(E) | Human Anti-FCGRT Recombinant Antibody; Fab Fragment (HPAB-0199-CN-F(E)) | FC | Humanized Fab |
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| HPAB-0201-CN-F(E) | Human Anti-FCGRT Recombinant Antibody; Fab Fragment (HPAB-0201-CN-F(E)) | ELISA, FC | Humanized Fab |
| HPAB-0202-CN-F(E) | Human Anti-FCGRT Recombinant Antibody; Fab Fragment (HPAB-0202-CN-F(E)) | FC | Humanized Fab |
| HPAB-M0088-YC-F(E) | Human Anti-FCGRT Recombinant Antibody (clone FnAb8); Fab Fragment | ELISA | Human Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| PABC-246 | Human Anti-FCGRT Recombinant Antibody (PABC-246) | ELISA, FC, Block | Human IgG |
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| CAT | Product Name | Application | Type |
|---|---|---|---|
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|---|---|---|---|
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| CAT | Product Name | Application | Type |
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| VS-0425-YC338 | Recombinant Anti-FCGRT Vesicular Antibody, EV Displayed (VS-0425-YC338) | ELISA, FC, Cell-uptake |
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